卵母细胞体外成熟时间对绵羊核移植效率的影响

Effects of in vitro Maturation Time of Oocytes on Sheep Nuclear Transfer Efficiency

[目的]为绵羊克隆试验中卵母细胞的体外成熟及去核时间提供参考。[方法]利用盲吸法结合荧光显微镜检查，对绵羊不同成熟时间卵母细胞去核的效率及其后续重构胚的发育进行对比。[结果]体外成熟培养19～21h的绵羊卵母细胞在成熟率上显著高于体外成熟培养16～18h（P〈0．05），在去核成功率上显著高于体外成熟培养22～24h（P〈0．05）；3个试验组在卵裂率、囊胚率上差异不显著（P〉0．05），但是体外成熟培养19～21h的试验组囊胚平均细胞数要显著高于其他2组（P〈0．05）。[结论]体外成熟培养19-21h的卵母细胞较适于作为受体细胞进行绵羊核移植，可以显著提高囊胚的质量。

[Objective] The study aimed to provide references for the time of oocyte maturation in vitro and enucleation in the course of sheep nuclear transfer （NT）. [ Method] Compared the effects of different maturation time of oocytes on enucleation efficiency and reconstructed embryo development by means of blind enucleation and fluorescence microscopy. [Result] Treatment of IVM （in vitro maturation）19 -21 h was significantly higher than IVM 16 - 18 h treatment in oocyte maturation rate （P 〈 0.05 ）and was significantly higher than IVM 22 -24 h treatment in enu-cleation rate （P〈0.05 ）. Three treatments had no significant difference in cleavage rate and blastocyst rate （P 〉 0.05 ）, but IVM 19 - 21 h treat- ment was significantly higher than the other 2 treatments in average cell number of blastocysts （P 〈 0.05 ）. [ Conclusion ] The appropriate in vitro maturation time of oocytes was 19 -21 h for sheep nuclear transfer, which could significantly improve the quality of blastocysts according to the cell number per blastocyst（P 〈 0.05 ）.