贲门腺癌和食管腺癌基因表达谱比较

Comparison of gene profiling between cardia and esophageal adenocarcinoma

目的:比较贲门腺癌(CAC)与食管腺癌(EAC)组织的基因表达谱。方法:收集贲门肠化生(CIM)和Barrett食管(BE)、CAC和EAC组织,用含有4 096条人类基因cDNA的芯片对其mRNA差异性表达进行比较,用组织芯片免疫化学染色法观测相同差异性表达趋势基因的蛋白产物在CAC和EAC组织中的表达情况。结果:CIM和BE差异表达的基因与CAC和EAC差异表达的基因比较,相同差异性表达趋势的基因有23条,其中上调17条,下调6条。对上调基因乳腺癌耐受蛋白(BCRP)、细胞凋亡蛋白抑制因子1(cIAP1)和下调基因基质金属蛋白酶2(MMP2)的蛋白产物进行组织芯片分析,CAC组织中BCRP和cIAP1蛋白阳性率均为80.0%,EAC组织中2者阳性率均为33.3%,CAC组织中2者阳性率高于EAC组织(P=0.018)。CAC组织中MMP2蛋白阳性率为40.0%,EAC组织中为55.5%,2者比较,差异无统计学意义(P=0.286)。结论:CAC与EAC在mRNA和蛋白质表达上差异明显,分子生物学特性各异,组织起源可能不同。

Aim： To explore the differences in gene profiling between cardia and esophageal adenocarcinoma by cDNA and tissue array.Methods：Tissue specimens of 10 cases of CAC,9 cases of EAC,7 cases of Barrett＇s esophagus（BE） and 6 cases of cardia intestinal metaplasia（CIM） were collected.cDNA microarray was used to screen the gene expression levels between CAC and EAC,CIM and BE,respectively,while immunohistochemical staining of the different formalin fixed paraffin-embedded tumor specimens was used to detect the proteins.Results： The differentially expressed genes between CAC and EAC were compared with those between CIM and BE,and 23 genes expressed in differential trends,of which 17 were up-regulated and 6 were down-regulated.Some differentially expressed genes including BCRP,cIAP1 and BCRP were validated by immunohistochemical staining of formalin fixed paraffin-embedded tumor specimens.The positive rates of BCRP,cIAPT were 80.0% in CAC,33.3% in EAC,and both of the positive rates in CAC were higher than those in EAC（P=0.018）.The positive rate of MMP2 in CAC was 40.0%,which had no difference from 55.5% in EAC（P=0.286）.Conclusion： The characteristics of gene expression in mRNA and protein level between CAC and EAC were different significantly,which indicated that CAC and EAC might not be the same origin.