全反式维甲酸对EC9706细胞体外增殖抑制及诱导凋亡作用

Effects of all-trans retinoic acid on proliferation and apoptosis of EC9706 cells in vitro

目的:观察全反式维甲酸(ATRA)对食管癌细胞体外增殖抑制及诱导凋亡的作用。方法:应用不同浓度(1×10-6、1×10-5、5×10-5mol/L)ATRA体外处理人食管癌细胞EC9706(ATRA1、ATRA2、ATRA3组),并设不加ATRA的对照组,倒置显微镜下观察细胞的生长状态,HE染色后光镜下观察细胞结构的改变,应用MTT法测定细胞生长抑制率,免疫组织化学技术检测凋亡相关蛋白Bax及Bcl-2的表达情况。结果:经ATRA处理后,ATRA3组与对照组比较,细胞生长速度减慢,群体倍增时间延长(P均<0.05);光镜下可见细胞形态趋向良性分化,细胞质内成熟细胞器增多,生长抑制率增加(P<0.05)。与对照组比较,3个ATRA组细胞Bax蛋白表达增加、Bcl-2蛋白表达下降(P<0.01),且以ATRA2、ATRA3组为明显(P<0.05)。结论:ATRA能有效抑制EC9706细胞增殖,其机制与Bax及Bcl-2蛋白表达调控有关。

Aim： To investigate the inhibition effects of all-trans retinoic acid（ATRA） on proliferation of human esophageal carcinoma cells EC9706 in vitro.Methods：EC9706 cells were processed by variant concentration of ATRA（1×10^-6,1×10^-5,5×10^-5mol/ L）.The cellular growth status was observed by using phase contrast microscope.The growth inhibition rate was determined and the growth inhibition rate was detected by using MTT method.The cell morphological alteration was observed after HE staining.The expressions of apoptosis-associated gene Bcl-2 and Bax were detected by immunohistochemical assay（SP technique）.Results： After being treated with ATRA,the EC9706 cells had the followingchanges.Compared with control group,the cellular growth rate of ATRA3 group significantly fell,the group doubling time prolonged,and the inhibition rate decreased（P〈0.05）.A series of cell morphological changes was observed,such as more monofilaments and more well-differentiated organelle in experimental groups than those of the control group.Compared with control group,Bax protein expression rate increased,Bcl-2 protein expression rate decreased in ATRA groups（P〈0.01）,especially those of ATRA2,ATRA3 group（P〈0.05）.Conclusion： ATRA could inhibit the growth of EC9707 cells in vitro,and its mechanisms may be related to the expressions of apoptosis-associated protein Bcl-2 and Bax.