乌司他丁对梗阻性黄疸大鼠肠氧化应激的影响

Effects of ulinastatin on intestinal oxidative stress of rats with obstructive jaundice

目的:探讨乌司他丁(UTI)对梗阻性黄疸肠氧化应激的影响。方法:取雄性SD大鼠72只,随机均分为假手术组(A组)、梗阻性黄疸组(B组)、UTI干预组(C组)。B组、C组采用胆总管结扎法建立梗阻性黄疸模型,C组从术后第1天开始腹腔注射UTI 40 000 IU/(kg.d),A组和B组注射等量生理盐水。术后3、5、7、10 d,每组各取6只大鼠,取肠组织测丙二醛(MDA)及超氧化物歧化酶(SOD)含量,血浆内毒素水平,光镜观察末端回肠黏膜形态学改变,用病理图像分析系统测量肠绒毛高度及黏膜厚度。结果:与A组比较,B组各时间点和C组术后5、7、10d时肠组织MDA含量及血浆内毒素水平升高,SOD含量降低(P均<0.01)。C组各时间点肠组织MDA含量及血内毒素水平较B组降低,SOD含量较B组升高(P均<0.05)。C组肠组织MDA和SOD含量、血浆内毒素水平与A组术后3 d时比较,差异无统计学意义(P>0.05)。B组术后第3天即见肠黏膜受损改变,随时间推移进行性加重;C组较B组肠黏膜病理改变明显减轻。B组各时间点及C组术后5、7、10 d时小肠绒毛高度、黏膜厚度均低于A组(P<0.01),C组则较B组升高(P<0.05),C组与A组术后3 d时比较,差异无统计学意义(P>0.05)。结论:UTI可通过抗肠氧化应激保护梗阻性黄疸大鼠肠屏障功能,减轻内毒素血症,且对早期病变效果更好。

Aim：To investigate the effects of ulinastatin（UIT） on intestinal oxidative stress of rats with obstructive jaundice.Methods：Seventy-two male SD rats were randomly and equally divided into sham operation group（Group A）,obstructive jaundice group（Group B） and UIT treatment group（Group C）.Rats in Group B and C were given choledochus ligation to establish the obstructive jaundice model.Group C was intraperitoneally injected UIT 40 000 IU/kg every day since the first day postoperation,while Group A and Group B were injected into equal amount normal saline.At Day 3,5,7 and 10 after operation,malondialdehyde（MDA） and superoxide dismutase（SOD） in the intestine tissue of six rats from every group were determined,plasm endotoxin were detected,the terminal ileum mucosa was observed under the light microscope,and the height of villus and mucosal thickness were examined with image analysis system.Results： Compared with those of Group A,MDA in the intestine tissue and the level of plasm endotoxin were higher,and SOD level was lower in Group B（P〈0.01）.Compared with those of Group B,MDA in the intestine tissue and the level of plasm endotoxin were lower,and SOD level was higher in Group C（P〈0.05）.The injury of intestinal mucosa was obriouse in Group B since Day 3 after operation,which aggravated with time changing,and it was lighter in Group C than that in Group B.The height of villus and mucosal thickness were shorter in Group B than that in Group A（P〈0.01）,higher in Group C than that in Group B（P〈0.05）;but there were no significant differences in indexes mentioned above between Group C and Group A at Day 3 after operation（P〉0.05）.Conclusion： UIT may protect intestinal mucosal barrier and reduce endotoxemia in obstructive jaundice by resisting intestinal oxidative stress,and it is especially beneficial for the early change.