硫酸庆大霉素生物检定结果准确度探讨

The discussion of magnamycin sulfate determination accuracy

探讨硫酸庆大霉素测定准确度,提高化验结果准确性。改变缓冲液PH值使测定样品的抑菌圈直径更清晰,减少误差。当缓冲液及培养基在PH9.0时,抑菌圈菌层边缘清晰、圆整,抑菌圈与菌层的反差非常明显,减少测量误差。检验结果与原方法比较抑菌圈清晰、直径大小符合规定,重现性好、效价测定与原方法无显著性差异。

The discussion magnamycin sulfate determination accuracy, enhances the chemical examination result accuracy.. The change buffer solution PH value causes the determination sample the bacteriostasis circle diameter to be clearer, reduces the error. When buffer solution and culture medium when PH9.0, bacteriostasis circle fungus level edge clear, circle entire, the bacteriostasis circle and the fungus level contrast is extremely obvious, reduces the measuring error.. The examination result and the original method comparison bacteriostasiscircle clear, the diameter size conforms to the stipulation, reappears the nature to be good, the effect price determination and the original method does not have the difference obviously.