HLA-DR基因与肝细胞癌的相关性研究

Study on the correlation between HLA-DR alleles and hepatocellular carcinoma

目的探讨中国HBV感染人群HLA-DR基因与肝细胞癌的相关性。方法应用PCR-SSP方法对102例慢性乙型肝炎患者(实验1组)、38例肝硬化患者(实验2组)、34例肝细胞癌患者(实验3组)及1383例中华骨髓库山东分库无血缘关系自愿骨髓捐献者(对照组)的HLA-DR等位基因进行检测,分析其表达与肝细胞癌的相关性。结果HLA-DR1在实验3组中的分布频率较对照组明显增高(χ2=4.80,P<0.05),HLA-DR13在实验3组中的分布频率较实验1组明显增高(χ2=5.56,P<0.05),实验2组与实验3组比较差异均无统计学意义。结论HLA-DR1可能是肝细胞癌的易感基因,携带HLA-DR13基因位点的HBV感染者更易发展为肝细胞癌,HLA-DR各等位基因表型频率在肝硬化和肝细胞癌患者中的分布无差异。

Objective To investigate the association between HLA-DR alleles and hepatocellular carcinoma. Methods Experiment groups were composed of 102 patients with chronic hepatitis B （groupl）, 38 patients with liver cirrhosis （group2） and 34 patients with hepatocellular carcinoma （ group3 ）. The control group was composed of 1383 marrow donors from China Marrow Bank Shandong Branch. HLA-DR were typed by PCR- SSP. Results HLA-DR1 were significantly more frequent in group3 compared with the control group （X^2 = 4. 80, P 〈 0.05 ）, HLA-DR 13 was significantly more frequent in group 3 compared with groupl （X^2 = 5.56, P 〈 0. 05 ）, there was no significant difference between group 2 and group 3. Conclusion The HLA-DR 1 might be associated with hepatocellular carcinoma. Patients with HBV infection,who carried HLA-DR13 allelie genes had hepatoeellular carcinoma easier. All kinds of HLA-DR allelic genes had no significantly different frequency between patients with liver cirrhosis and those with hepatocellular carcinoma.