药物干预对大鼠脑缺血后神经再塑及功能恢复的实验研究

The effect of methyphenidate on transient cerebral ischemia in rats after nerve plasticity and functional recovery

目的研究大鼠脑缺血后,损伤区域及相关部位的神经再塑与运动行为恢复之间的相互关系;以及哌醋甲酯对神经再塑以及肢体功能恢复的影响。方法线栓法建立大鼠的大脑中动脉缺血模型,术后第3天起,对手术给药组及假手术给药组的大鼠口服灌注哌醋甲酯;于术后第7、14、21、28天,触觉刺激试验作行为测试,记录其撕去胶布所使用的时间。采用免疫组织化学方法观察突触素(SYN)及突触后致密物质(PSD-95)在大鼠皮层缺血灶的内侧、外侧、对侧的表达。结果SYN及PSD-95的免疫反应产物于术后第7天在3个观察部位都显著减少,在14d以后明显增加,与此相对应的肢体活动在第21天明显恢复。手术给药组前肢功能恢复较之假手术给药组及手术对照组于第14天即有显著进步(P<0.01),至第28天甚至超过假手术对照组(P<0.05)。结论哌醋甲酯对大鼠脑缺血后神经再塑以及肢体功能恢复有促进作用。

Objective To observe the effect of methyphenidate to the motor function of forelimbs and the distribution and density of SYN and PSD-95 after cerebral ischemia in rats. Methods Unilateral cerebral ischemia was induced in rats by the middle cerebral artery occlusion （MCAO）. Recovery times were 7,14,21, 28 days postoperatively. MPH were given to the rat belong SD and OD each day. Forelimbs function was evaluated with tacticl stimulation test. Synaptophysin and PSD-95 were examined by immunohistochmical techniques. Results Both synaptophysin and PSD-95 demonstrated statistically significant increase in the density of immunoreaction in the neocortical regions medial and lateral to the infarction as well as in the contralateral neocortex at 14th day compared with sham controls. Meanwhile, forelimbs functional recovery has statistically significant increased at 21th day. Conclusion The functional recovery of forelimbs and the distribution and density of SYN and PSD-95 after cerebral ischemia in rats is accelerated by methyphenidate treatment.