不同年龄肋软骨的组织形态学及Ⅱ型胶原蛋白含量变化的研究

Histologic study and type Ⅱ collagen expression in the rib cartilage of patients at different age

目的探讨肋软骨的组织形态学及Ⅱ型胶原蛋白在不同年龄阶段的变化规律。方法将2005年至2006年行耳廓再造的患者按年龄不同分为3组。5～10岁为儿童组，11～17岁为青少年组，18—29岁为成人组，每组30例。观察不同年龄组肋软骨的组织形态学变化及Ⅱ型胶原免疫组织化学染色的定量分析，采用MoticMed6．0A数码医学图像分析系统进行免疫组织化学图像定量测定，测量指标为平均积分光密度，对分析结果进行统计学处理。结果儿童组肋软骨膜血管最丰富，软骨基质染色均匀，软骨细胞数目最多，Ⅱ型胶原蛋白表达最活跃，平均积分光密度值最高；青少年组软骨膜内血管减少，软骨基质染色出现明显的不均质状，软骨陷窝体积变大，并呈分隔状，陷窝内软骨细胞数目减少，Ⅱ型胶原蛋白表达较儿童组减弱；成人组软骨膜血管、细胞成分明显减少，软骨膜内的纤维成分明显玻璃样变，钙盐沉积较青少年组时明显增多，Ⅱ型胶原蛋白表达较青少年组减弱。经统计学分析，3组间差异有统计学意义（P〈0．01）。结论肋软骨的组织形态学随年龄增长发生变化，Ⅱ型胶原蛋白含量随年龄增长呈递减趋势。

Objective To study the difference of histologic examination and type Ⅱ collagen expression in the rib cartilage of patients at different age. Methods The patients were divided into three groups, as children group（5 to 10 years old）, teenager group（ 11 to 17 years old）, and adult group（ 18 to 29 years old）. Histologic examination of rib cartilage and quantitative analysis of type Ⅱ collagen with immunohistochemical stain were carried out in the three groups. Motic Med 6.0 A system was used to quantitate immunohistochemical image through measurement of mean photodensity. The results were statistically analyzed. Results Among the three groups, the children＇s rib cartilage had the most blood vessels, the most chondrocytes, well-distributed stain of matrixes, and the type Ⅱ collagen was expressed actively and highest in photodensity. The rib cartilage of teenager group had less blood vessels, unhomogeny distributed stain of matrixes, the enlarged and separated cartilage lacunas. The rib cartilage in adult group showed the least blood vessels, the least chondrocytes, the hyalinization of perichondium, the most deposition of calcium salt, and the type Ⅱ collagen was expressed at the lowest level in photodensity. Statistical analysis showed significant differences among the three groups （P 〈 0.01 ）. Conclusions Histologic stmcture in human rib cartilage changes during the aging process and the type Ⅱ collagen is decreased with the increase of age.