家蚕胚胎期重力相关基因的抑制消减杂交分析

Suppression Subtractive Hybridization Analysis of Gravity Related Genes of Silkworm Embryogenesis

目的:筛选家蚕胚胎期重力相关基因。方法:对模拟失重与正常重力条件下的家蚕胚胎cDNA进行抑制消减杂交(suppression subtractive hybridization,SSH),并对模拟失重过程中家蚕胚胎期表达发生变化的基因进行克隆、测序及同源性分析。结果:获得了34个与重力有关的序列标签。在模拟失重条件下有16个基因表达上调,其中15个为未知基因,1个为已知基因,其作用是维持mRNA的稳定性。在模拟失重条件下有18个基因表达下调,其中4个为未知基因,6个为蛋白合成相关基因,3个为基因组contig基因,5个为家蚕est库中功能未知基因。结论:模拟失重环境影响了家蚕胚胎发育期与mRNA稳定性和蛋白质合成相关基因的表达。

Objective： To screen gravity related genes of silkworm Embryogenesis. Methods： Suppression subtractive hybridization （SSH） was used to isolate gravity related genes of silkworm embryogenesis in simulated weightlessness and normal gravity environment. Differential expression genes were cloned, sequenced and analyzed on homology. Results： 34 gravity related genes were obtained using SSH. 16 genes were up regulated in simulated weightlessness environment, including 15 unknown genes and one known gene maintaining the stability of mRNA. 18 genes were down regulated in simulated weightlessness environment, including 3 unknown genes, 4 contig genes, 5 submitted and unknown function genes in SilkBase, and 6 protein synthesis genes. Conclusion： Simulated weightlessness environment affects the expression of genes playing a role in maintaining the stability of mRNA and synthesizing protein during the period of silkworm embryogenesis.