富勒烯对人胚肝细胞L-02的损伤作用

Human Embryo Liver L-02 Cells Damage Induced by Fullerene

目的探讨富勒烯(C_(60))对人胚肝细胞(L-02)的毒性作用及其可能的作用机制。方法将L-02细胞液分别暴露于0、1.25、2.5、5、10、20、40μg/ml的C_(60)悬浊液中染毒24 h后,检测细胞内LDH、GSH含量和SOD活力,并比较不加和加入抗氧化剂N-乙酰半胱氨酸(NAC)条件下的细胞存活率。结果与空白对照组相比,1.25、2.5、5、10、20、40μg/ml C_(60)单独染毒组细胞存活率较低,差异均有统计学意义(P<0.05或P<0.01);且呈现明显的剂量-效应关系。加入抗氧化剂NAC后,1.25、2.5、5、10μg/rnl联合染毒组细胞存活率有所上升,差异均有统计学意义(P<0.05或P<0.01)。与空白对照组相比, 1.25、5、10、20、40μg/ml C_(60)染毒组LDH活力较高,5、20、40μg/ml C_(60)染毒组SOD活力和10、20、40μg/ml C_(60)染毒组GSH含量降低,差异均有统计学意义(P<0.05或P<0.01)。结论氧化损伤可能是C_(60)对L02细胞毒性作用的机制之一。

Objective To study the toxicity and mechanism of fullerene （C60）on human embryo liver L-02 cells. Methods L-02 cells were exposed to C60 suspension of different concentrations （0.00, 1.25, 2.50, 5.00, 10.00, 20.00 and 40.00 ug/ml） for 24 h, then the content of GSH and the activity of LDH, SOD were determined, the viability of cells with/without NAC was also compared. Results Compared to the control group, the viability of cells exposed to 1.25, 2.50, 5.00, 10.00, 20.00 and 40.00 ug/ml C60 suspension decreased in a dose-dependent manner, and the differences were significant （P〈0.05 or P〈0.01）. In C60-NAC joint exposure groups, the viability of cells increased in degree in groups of 1.25, 2.50, 5.00 and 10.00 ug/ml, and the differences were significant （P〈0.05 or P〈0.01）. Compared with the control group, cellular LDH activity increased in 1.25, 5.00, 10.00, 20.00 and 40.00 ug/ml groups, SOD activity in 5.00, 20.0, 40.0 ug/ml groups and GSH contents in 10.00, 20.00, 40.00ug/ml groups decreased significantly （P〈0.05 or P〈0.01）. Conclusion C60 can cause L-02 cells damage and oxidative injury should be concerned in the possible mechanism.