类风湿性关节炎患者抗天然Ⅱ型胶原抗体的测定及临床意义

Measurement of anti native type Ⅱ collagen antibody in patients with rheumatoid arthritis and its clinical significance

以本科提纯的天然Ⅱ型胶原（ＮａｔⅡ）为抗原，以新鲜羊血清平衡液为封闭液，用酶联免疫方法检测８８例类风湿性关节炎（ＲＡ）病人和８２例健康对照者血清中抗ＮａｔⅡ抗体水平。结果：ＲＡ病人血清抗ＮａｔⅡ抗体阳性率为：ＩｇＧ３６．３６％、ＩｇＭ１９．３２％；健康对照者分别为：４．８８％及１．２％。抗ＮａｔⅡ抗体ＩｇＧ阳性的ＲＡ患者血小板升高，类风湿因子阳性及关节Ｘ线分期较高者均多于阴性的ＲＡ患者（Ｐ＜０．０５）。在抗ＮａｔⅡ抗体ＩｇＭ阳性组仅血小板升高者多于阴性组（Ｐ＜０．０１）。ＲＡ病人的抗ＮａｔⅡ抗体阳性率高于健康对照者，提示对ＮａｔⅡ的自身免疫在ＲＡ的发病机制中可能起一定作用。

The native type Ⅱ collagen purified by our laboratory was used in coated microtiter plate and buffered normal goat serum(BNGS) as a block solution to detect the anti Nat Ⅱ Ab level by means of ELISA method in 88 rheumatoid arthritis (RA) patients serum samples and 82 normal controls.Results:In RA patients the anti-Nat Ⅱ Ab positive rates of IgG isotype was 36.36%, and of IgM isotype was 19.32%. In normal controls, the IgG isotype, and of IgM isotype was 4.88% and 1.2% respectively. It was found that in anti Nat Ⅱ Ab IgG positive group of RA patients′ platelets, rheumatoid factor and joint X ray grade were higher than negative group of RA patients′,in anti Nat Ⅱ Ab IgM positive group of RA patients,only the amount of platelets was higher than that in the negative group.The fact that anti Nat Ⅱ Ab positive rate of RA patients was higher than that of normal controls that indicated the autoimmue to Nat Ⅱ Ab plays a potential role in pathogenesis of RA. Author′s address\ Dept.of Immunology, China Japan Friendship Hospital, Beijing, 100029