以阳性水平指数作为肺癌p53表达水平指标的探讨

The positive level index as reflector of p53 expression in pulmonary carcinoma

目的：探讨一种能全面、客观、准确地评价ｐ５３的表达水平的指标。方法：用免疫组化ＡＢＣ法检测４５例肺癌组织的ｐ５３蛋白表达，用显微图像分析仪测定阳性细胞百分率Ｐ／Ａ（％）、平均光密度（ＡＯＤ），并将前两者的乘积作为阳性水平指数（ｐｏｓｉｔｉｖｅｌｅｖｅｌｉｎｄｅｘ，ＰＬＩ）。结果：ｐ５３表达阳性率在肺鳞癌及肺腺癌分别为５０％、５２．２％。肺鳞癌阳性水平指数略高于肺腺癌，但两者之间差异无显著性。结论：阳性水平指数能较全面准确地反映免疫组化反应的水平，有利于不同免疫组化反应进行比较。

Purpose To find a comprehensive and accurate reflector of immunohistochemical response level. Methods Immunohistochemical techniques were used to study the expression of p53 in 45 cases of pulmonary carcinoma. The percentage of positive cell P/A(%) 、average optical density (AOD) were assessed by image analyser and positive level index (PLI)=P/A(%)×AOD. Results The positivity rate of p53 expression in squamous cell carcinoma and adenocarcinoma were 50% and 52 2%, respectively. The PLI of squamous cell carcinoma was higher than that of adenocarcinoma, but there was no significant correlation. Conclusions The PLI can be used as a comprehensive and objective reflector of immunohistochemical response level and it is convienent to compare the studies of immunohistochemistry.