人重组骨形成蛋白-1对牙髓细胞生物学效应的研究

The Biological Effects of Recombinant Human Osteogenic Protein1 to Cultured Pulp Cells

通过细胞培养技术、噻唑盐比色测定（ＭＴＴ）、酶动力学方法和放射免疫技术，了解人重组骨形成蛋白－１（ｒｈＯＰ－１）对体外培养的人牙髓细胞增殖、碱性磷酸酶活性及骨钙素表达的影响。结果表明，ｒｈＯＰ－１可促进牙髓细胞增殖，提高牙髓碱性磷酸酶活性及骨钙素的表达，在本实验剂量范围内呈浓度依赖关系，具有诱导牙髓细胞向成骨方向转化的趋势。

The study was to observe the biological effects of rhOP1 on the proliferation, ALPase activity and osteocalcin of dental pulp cells by cell culture technique, MTT, enzyme kinetic methods and radioimmunoassay. The results indicated rhOP1 could obviously promote the proliferation and enhanced ALPase activity and osteocalcin secration of pulp cells with showing dosedependent relationship in this experimental dose range. Thus, rhOP1 can induce dental pulp cells to differentiate the cells expressing osteoblast phenotype.