涎腺腺样囊性癌间质成分的电镜组织化学及免疫组织化学研究

Study of Electron Microscopy Histochemistry and Immunohistochemistry of Extracellular Matrix in Adenoid Cystic Carcinoma

采用Ⅳ型胶原及纤维连接蛋白的免疫组织化学技术研究２２例涎腺腺样囊性癌（筛孔型７例，管状型１５例），同时采用电镜组织化学技术研究４例筛孔型腺样囊性癌。结果表明，腺样囊性癌筛状结构中分布有复层基板、蛋白多糖和胶原原纤维。免疫组化显示，筛孔中的间质成分富含Ⅳ型胶原和纤维连接蛋白。在管状型肿瘤中，小梁和条索周边的间质成分也富含Ⅳ型胶原及纤维连接蛋白。因此提示，Ⅳ型胶原及纤维连接蛋白是腺样囊性癌的主要间质成分，可能由肿瘤性肌上皮细胞分泌产生。

In order to determine the character of the extracellular matrix in adenoid cystic carcinoma (ACC), 22 cases of ACC of salivary gland were examined by immunohistochemical methods and 4 cases were examined by electron microscopy histochemically. Immunohistochemistry, type Ⅳ collagen and fibronectin were more intensively localized in pseudocyst space and in extracullular matrix between tumor cells. Ultrastructrual study showed that the pseudocystic cells had abundant rough endoplasmic reticulum and a welldeveloped Golgi apparatus, some cells had muscle microfilament. There were laminin, proteoglycan and collagen fibril in the pseudocyst space. The results indicated that type Ⅳ collagen and fibronectin were the important stromal component in ACC, which may be secreted by neoplasmic myoepithelial cells.