Cdc25 Ccyclin B1在二烯丙基二硫化物诱导人胃癌BGC823细胞G_2/M期阻滞中的作用

Cdc25C and Cyclin B1 are Involved in Cell Cycle G_2 /M Arrest Induced by Diallyl Disulfide in Human Gastric Cancer BGC823 Cells

目的:研究二烯丙基二硫化物(DADS)对人胃癌BGC823细胞的增殖及细胞周期的影响以及Cdc25C、cyclin B1的作用。方法:采用MTT法检测BGC823细胞的生长活性;流式细胞术分析DADS对细胞周期分布的影响;Western blot观察DADS对Cdc25C蛋白、cyclin B1蛋白表达的影响。结果:MTT比色实验结果显示,DADS对人胃癌细胞具有明显的生长抑制作用,且呈显著的剂量-效应依赖关系(P<0.05)。流式细胞分析发现,DADS作用12h时,G2/M期细胞增加到30.1%;24h时增加到58.1%;36h达50.2%(P<0.05)。Western blot结果表明,DADS呈时间依赖性抑制人胃癌BGC823细胞周期Cdc25C磷酸酶的表达,cyclinB1表达在DADS处理24h后开始下降(P<0.05)。结论:DADS可抑制人胃癌BGC823细胞增殖,其增殖抑制与细胞周期G2/M期阻滞有关;DADS可能是通过抑制Cdc25C、cyclinB1表达使部分BGC823细胞停滞在G2/M期。

Objective： To investigate the effect of diallyl disulfide （DADS） on the growth and cell cycle of human gastric cancer cell line BGC823 and to explore the role of Cdc25C and cyclin B1 in this process. Methods： The growth inhibition of BGC823 cells was measured by MTT assay. Phase distribution of cell cycle was analyzed by flow cytometry. The expression of Cdc25C and cyclinB1 was determined by Western blot. Results： DADS inhibited the growth of human gastric cancer BGC823 cells. Flow cytometry analysis revealed that BGC823 cells treated with DADS presented with increasing percentages of cells in the G2/M phase as time went by. The proportion of cells in the G2/M in cells treated with 15mg/L DADS for 24 h was more than three times than that of untreated cells. Western blot analysis showed that DADS decreased the level of Cdc25C in BGC823 cells in a time-dependent way （P〈0.05）. The expression of cyclin B1 was decreased by DADS after 24h （P〈 0.05）. Conclusion： DADS can inhibit Cdc25C and cyclin B1 and thus are involved in the growth inhibition and G2/M arrest of BGC823 cells.