胶原寡聚肽保湿抗皱功效评价及其机制的初步探讨

Evaluating the capacity and investigating the mechanism of collagen oligopeptide on holding water and resisting wrinkle

目的:通过人体试验评价胶原寡肽保湿抗皱功效,并对其机制进行初步探讨。方法:采用随机、双盲、对照的实验方法,对含有胶原寡肽成分的眼霜其保湿、抗皱效果进行临床效果评价;并用体外试验方法确定胶原寡肽对成纤维细胞增殖活性的影响。结果:与基础值相比,含有胶原寡肽成分的眼霜在第1、4周,可以显著地增加皮肤角质层的含水量,改善眼周皱纹;体外试验证实,胶原寡肽处理细胞后96、120h,成纤维细胞增殖活性显著增加,以1.00mg/ml组作用最显著。结论:在细胞水平,胶原寡肽可以促进成纤维细胞增殖;胶原寡肽做为化妆品的添加成分能够提高化妆品的保湿抗皱效果,但是其透皮吸收性及长期效果有待于进一步证实和提高。

Objective Verifying the function of collagen oligopeptide on holding water and resisting wrinkle in vivo and investigating its mechanism. Methods The random, double-blind and controlled method was used to evaluate the effects of the collagen oligopeptide on holding water and resisting wrinkle. The ability of the proliferation of the cultured cells was measured by WST-8. Results The effects of moisturizing and resisting wrinkle of collagen oligopeptide are significant compared with the baseline at the first, forth week. After treated by the collagen oligopeptide,the number of the cultured cell are more than the controlled group at 96, 120h significantly, especially in the group of 1.00 mg/ml. Conclusion The proliferation of the fibroblast can be boosted by the collagen oligopeptide, and the effect of moisturizing and resisting wrinkle of cosmetics can be improved by adding collagen oligopeptide, but the percutaneous absorption and the long term effect needed to be further verified.