survivin反义核酸诱导HeLa细胞凋亡的实验

Apoptosis in HeLa Cells Induced by Antisense Oligodeoxynucleotide of Human survivin Gene

目的探讨survivin反义核酸诱导HeLa细胞发生凋亡的可能性,揭示该凋亡发生与Bcl-2和Bax之间的关系。方法采用TUNEL染色法,研究survivin反义核酸与HeLa细胞凋亡的关系;通过免疫组织化学法检测凋亡相关基因Bcl-2和Bax的表达。结果survivin反义核酸在体外能诱导HeLa细胞凋亡,下调Bcl-2的表达,增强Bax的表达。结论诱导HeLa细胞凋亡是survivin反义核酸抗HeLa作用的机制之一,survivin反义核酸可能通过下调Bcl-2的表达,增强Bax的表达,诱导HeLa细胞发生凋亡。

Objective To investigate the apoptosis in HeLa cell induced by antisense oligodeoxynucleotide of human survivin gene, and the relation between this apoptosis and expression of Bcl-2 and Bax. Methods In vitro experiments, TUNEL staining method was used to detect the apoptosis status of cervical cancer HeLa cell line treated by antisense oligodeoxynucleotide of survivin. Immunohistochemical staining was used to detect the expression of apoptosis-relation gene Bcl-2 and Bax. Results Antisense oligodeoxy nucleotide of survivin was able to induce the apoptosis in HeLa cells. Antisense oligodeoxynucleotide of survivin can reduce the expression of apoptosis-relation gene Bel-2 and increase the expression of apoptosisrelation gene Bax. Conclusion Antisense oligodeoxynueleotide of survivin was able to induce the apoptosis in HeLa cell. This apoptosis may be mediated by down-regulation of apoptosis-regulated gene Bcl-2 and up-regulation of Bax.