摘要
目的探讨转酮酶样基因TKTL1在人类结肠癌发生发展中的作用。方法用实时定量PCR检测结肠癌及癌旁组织中转酮酶样基因TKTL1 mRNA表达水平,连续监测法检测各组织中总转酮酶活性。结果TKTL1 mRNA在结肠癌组织中比癌旁组织中的表达明显增强,且侵袭性结肠癌比非侵袭性结肠癌表达增强。同样,结肠癌组织中总转酮酶活性比癌旁组织中高(P<0.01),侵袭性结肠癌较非侵袭性结肠癌中转酮酶活性增高(P<0.01)。结论TKTL1 mRNA表达与人类结肠癌的发生发展密切相关。
Objective To explore the effect of TKTL1 on occurrence and development of colon cancer. Methods Real time PCR was used to determine the mRNA expression of TKTL1 gene in human colon cancer and para-cancer tissue. Continuous monitoring assay was used to determine total transketolase activity in the human colon cancer and para-cancer tissue. Results The expression of TKTL1 gene was significantly higher in the colon cancer tissues than para-cancer tissue. Further more, the expression of TK-TL1 gene was significantly up-regulated in the invasive colon cancer compare with the noninvasive colon cancer. The total transketolase activity significant increase in the colon cancer tissues compare with paracancer tissue. The total transketolase activity significant increase in the invasive cancer tissue cornpare with the noninvasive colon cancer. Conclusion Expression of TKTL1 mRNA is related to the occurrence and development of human colon cancer.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2008年第11期812-814,共3页
Cancer Research on Prevention and Treatment
