新扬州鸡IGF-Ⅰ基因多态性的PCR-SSCP检测及其与生长发育性状的相关性

Detection of IGF-Ⅰ gene mutation with PCR-SSCP and the association between IGF-Ⅰ gene polymorphisms and growth character

以新扬州鸡胰岛素样生长因子-Ⅰ(IGF-Ⅰ)基因作为控制其生长、发育性状的候选基因,通过对IGF-Ⅰ基因主要部分序列进行PCR-SSCP检测,发现了2个多态位点.测序结果表明,IGF-Ⅰ基因存在2个突变位点:插入(G)和(G→C).分析多态位点与0-12周龄鸡生长发育性状的关联性,结果表明:插入(G)突变基因型中,2周龄鸡体重BB型与AB型差异显著(P<0.05),但AA型与AB型和BB型差异均不显著,而(G→C)突变各基因型间没有显著差异;插入(G)突变位点对0-12周龄鸡的龙骨长、胫骨长均没有显著影响,而(G→C)突变位点仅在鸡6周龄时表现出与龙骨长、胫骨长间的差异显著性.

Due to the importance of insulin-like growth factor-Ⅰ （IGF-Ⅰ） in regulating many physiological and metabolic processes in animal body, we applied PCR-SSCP to detect the mutation of IGF-Ⅰ gene in New Yangzhou chickens and to analyze DNA sequence. We gained two mutation loci of IGF-Ⅰ gene ： insert （ G）, （G→C）. The association between DNA mutation loci and chicken traits was analyzed with general line model. Tile results were as follows. In the insert （G） mutation genotypes, there was significant difference between body weights of 2 weeks aged BB and AB genotype（P 〈 0.05）, while there was no significant difference between AA and other genotypes. In the （G→C） mutation genotypes, there was no significant difference in body weight. Insert （G） mutation loci had no significant effect on fossil bone length and shank length of 0 -12 weeks aged chickens, only （G→C） mutation loci had a significant effect on fossil bone length and shank length of 6 weeks aged chickens.