摘要
通过二维聚丙烯酰胺凝胶电泳以及计算机辅助的图像分析技术,对荔枝开花后40 d的正常与败育胚蛋白质图谱进行初步分析。结果表明,100个蛋白质点在表达丰度上有明显差异;选择仅在正常发育胚胎胶上表达的蛋白点15个和仅在败育胚胎胶上表达的蛋白点50个,进行基质辅助激光解吸附电离飞行时间质谱(MALDI-TOF-MASS)分析,鉴定出9个与胚发育相关的蛋白,这些蛋白可能参与了胚败育的调节和控制。
The global protein patterns in normal and aborted embryo of litchi at 40 days after flowering （DAA） were compared by using two-dimensional polyacrylamide gel electrophoresis （2-DE） and computer image analysis. The results showed that the quantities of 100 protein spots in normal embryo were significantly different from aborted embryo. There were 15 and 50 spots appeared on the gel of normal embryo and aborted embryo, respectively. Then they were selected for MALDI-TOF-MASS analysis. Nine proteins related with embryo development were identified, which maight involve in regulating and controlling the embryo abortion.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2008年第6期537-544,共8页
Journal of Tropical and Subtropical Botany
基金
863计划项目(2004AA001380)
海南省自然科学基金项目(80419)
海南省教育厅高校科学研究项目(HJKJ200740)
海南师范大学植物学重点扶持学科项目资助
关键词
荔枝
胚发育
蛋白质图谱
质谱
Litchi
Embryo development
Proteomics mapping
MALDI-TOF-MASS
