犬细小病毒核酸疫苗、重组活载体疫苗与弱毒疫苗免疫犬实验研究

Experimental Study on Dogs Immunized with CPV DNA Vaccine,CPV Recombinant Vector Vaccine and CPV Modified Vaccine

分别用犬细小病毒(CPV)核酸疫苗(pVCPV-VP2)、CPV重组活载体疫苗(CAV2/CPV)与CPV弱毒疫苗对犬进行了免疫试验,以检测不同CPV疫苗的免疫原性。采用CPV ELISA、CPV HI与CPV微量中和试验检测免疫犬的体液免疫水平,采用淋巴细胞转化试验检测犬的细胞免疫水平。结果,pVCPV-VP2和CAV2/CPV均能诱导机体产生抗CPV ELISA抗体与抗CPV中和抗体,但是pVCPV-VP2不能诱导机体产生可检测的抗CPV HI抗体,而CAV2/CPV能够诱导机体产生抗CPV HI抗体。淋巴细胞转化试验结果,pVCPV-VP2和CAV2/CPV免疫犬的外周血淋巴细胞对ConA与CPV的刺激均出现明显的增殖反应。结果表明,pVCPV-VP2和CAV2/CPV免疫犬均能诱导机体产生抗CPV的特异性体液免疫反应和细胞免疫反应,两者所表达的VP2蛋白均具有较好的免疫原性。CAV2/CPV以及pVCPV-VP2和CAV2/CPV联合免疫犬的抗CPV体液免疫水平和细胞免疫水平均比用pVCPV-VP2单独免疫犬的体液免疫水平和细胞免疫水平高。但CAV2/CPV诱导机体产生的抗CPV特异性免疫反应仍然比CPV弱毒疫苗诱导机体产生的抗CPV特异性免疫反应弱。另外,CAV2/CPV还能诱导机体产生抗CAV-2的特异性免疫反应。

To test the immunogenicity of the different CPV vaccines, the dogs were respectively vaccinated with pVCPV-VP2, CAV2/CPV and CPV modified vaccine. The antibody titers against CPV were detected by CPV ELISA, CPV HI and CPV SN. And the proliferation of the PBMCs of the vaccinated dogs was detected by MTT. As a result, both anti-CPV ELISA anti- body and SN antibody were checked up on in the dogs vaccinated with CAV2/CPV or pVCPV- VP2. Anti-CPV HI antibody was discovered in the dogs immunized with CAV2/CPV or CAV2/ CPV combined with pVCPV-VP2, but not in the dogs vaccinated with pVCPV-VP2. And the PBMCs of the inoculated dogs obviously proliferated against both CPV and ConA stimulation, respectively. It demonstrated that CAV2/CPV and pVCPV-VP2 induced humoral and cellular im- munity against CPV in dogs. The immune levels elicited by CAV2/CPV or CAV2/CPV combined with pVCPV-VP2 were higher than that by pVCPV-VP2, but weaker than that by the CPV modified vaccine. At the same time, CAV2/CPV can also induce specific anti-CAV-2 immunity.