安徽地区绝经后妇女细胞色素P450基因CYP17多态性与骨密度及血脂相关性研究

Study on association between CYP17 polymorphisms in cytochrome P450c(P450) gene and bone mineral density and serum lipid in Anhui postmenopausal women

目的了解安徽地区汉族绝经后女性的P450基因CYP17多态性分布与骨密度及血脂的相关性。方法随机选择194名安徽合肥地区健康汉族绝经后妇女,运用双能X线骨密度吸收法测定腰椎L2-L4和股骨近端部位的骨密度(BMD),并采用聚合酶链反应—?限制性片断长度多态性方法对P450基因的CYP17多态性位点进行基因分析。测定血甘油三酯(TG)总胆固醇(TC),低密度脂蛋白(LDL)。结果①CYP17基因型分布频率依次为TC型,CC型,TT型;②CYP17位点TC型Ward s三角区BMD明显高于CC型(P<0.05),与腰椎以及股骨颈和大转子区BMD无关(P>0.05)。在调整年龄、身高、体重、BMI、绝经年限等因素后,CYP17多态位点与Ward s三角区BMD显著相关(P<0.05)。③血TC与股骨颈BMD呈负相关(r=-0.210,P=0.015),LDL与腰椎BMD呈显著负相关(r=-0.225,P=0.009),TG与股骨颈BMD呈显著负相关(r=-0.275,P=0.001),与Ward s三角区BMD呈负相关(r=-0.174,P=0.043)。各基因型间血脂水平无差异(P>0.05)。结论①安徽地区绝经后女性P450基因CYP17多态性位点与绝经后女性Ward s三角区BMD有相关性;②血TC、LDL、TG升高可能是绝经后女性骨质疏松发生的危险因素;③未发现P450基因CYP17多态性位点与安徽地区绝经后女性血脂水平有相关性。

Objective To investigate the distribution of CYP17 polymorphisms in eytoehrome P450c（P450） gene in postmenopausal Han women in Anhui as well as its con＇elation with bone mineral density（BMD） and serum lipid level. Methods CYP17 polymorphism of P450 gene were analyzed by polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）. BMD at lumbar spine 2-4 and the femoral neck, Ward＇s triangle and trochanter were measured by dual-energy X-ray absorptionmetry （DEXA）. Triglyceride （TG）, total cholesterin （TC） and low-density lipoprotein （LDL） was measured from blood samples. Results O The distribution of CYP17 genotypes in postmenopausal Han women in Anhui was as follows： TC, CC, TT. （2）BMD at the Ward＇s triangle was significantly lower in subjects with CYP17 CC genotype than in the combined group with TC genotype （ P 〈 0.05 ）, no association with BMD at lumbar spine 2-4 and the femoral neck and trochanter （ P 〉 0.05 ）. （3） No association was found between serum lipid and any genotype of CYP17 （ P 〉 0.05 ）. Conclusions （1） CYP17 polymorphism in P450 gene may have an influence on BMD in postmenopausal women. （2）The increased TC, LDL and TG may be involved in the pathogenesis of postmenopausal osteoporosis. （3）No association is found between CYP17 polymorphism in P450 gene and serum lipid in postmenopausal women in Anhui.