摘要
目的:研究我国宫颈癌高发区新疆维吾尔族妇女宫颈癌与人类乳头状瘤病毒(HPV)感染的关系。方法:对65例新疆维吾尔族妇女的宫颈癌活检组织标本,应用原位杂交法检测HPV6/11、16/18和31/33/35DNA;L1共有序列引物聚合酶链反应(PCR)及E6特异型引物PCR检测其中的58例HPV6、16和18DNA。结果:65例宫颈癌患者中,原位杂交法检测HPVDNA有28例阳性(43.1%);L1PCR和E6PCR检测HPVDNA阳性分别为13例(22.4%)和45例(77.6%)。结论:对宫颈癌标本中的HPVDNA检测,E6特异性引物PCR敏感性最高,L1PCR及原位杂交相对较低。新疆维吾尔族妇女宫颈癌发病与HPV感染有密切关系。
Objective: To study the association of cervical cancer with human papillomaviral (HPV) infection in Uygur women at high risk for development of cervical cancer in Xinjiang. Method: Cervical samples from 65 Uygur women with cervical cancer were collected. HPV DNA types 6/11,16/18,31/33/35 were studied by in situ hybridization, and both consensus and type specific primers for HPV types 6,16 and 18 were used in 58 cases by polymerase chain reaction (PCR). Results: HPV DNA was detected in 43.1%, 22.4% and 77.6% of specimens by the in situ hybridization, PCR using L1 consensus primers and E6 type specific primers, respectively Conclusions: E6 type specific PCR was more sensitive than L1 consesus PCR and in situ hybridization for detection of HPV DNA. These data supported the role of HPV DNA (particularly HPV 16) in the pathogenesis of cervical carcinoma in high risk women living in Xinjiang.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1997年第7期405-408,I039,共5页
Chinese Journal of Obstetrics and Gynecology
关键词
子宫颈肿瘤
HPV
DNA
病毒
PCR
活检
Cervix neoplasms
Papillomavirus
human
DNA
viral
Insitu hybridization Polymerase chain reaction