新疆维吾尔族妇女子宫颈癌活检组织中人类乳头状瘤病毒DNA的检测

Detection of Human Papillomavirus DNA in Cervical Squamous Cell Carcinoma in Xinjiang Uygur Women

目的：研究我国宫颈癌高发区新疆维吾尔族妇女宫颈癌与人类乳头状瘤病毒（ＨＰＶ）感染的关系。方法：对６５例新疆维吾尔族妇女的宫颈癌活检组织标本，应用原位杂交法检测ＨＰＶ６／１１、１６／１８和３１／３３／３５ＤＮＡ；Ｌ１共有序列引物聚合酶链反应（ＰＣＲ）及Ｅ６特异型引物ＰＣＲ检测其中的５８例ＨＰＶ６、１６和１８ＤＮＡ。结果：６５例宫颈癌患者中，原位杂交法检测ＨＰＶＤＮＡ有２８例阳性（４３．１％）；Ｌ１ＰＣＲ和Ｅ６ＰＣＲ检测ＨＰＶＤＮＡ阳性分别为１３例（２２．４％）和４５例（７７．６％）。结论：对宫颈癌标本中的ＨＰＶＤＮＡ检测，Ｅ６特异性引物ＰＣＲ敏感性最高，Ｌ１ＰＣＲ及原位杂交相对较低。新疆维吾尔族妇女宫颈癌发病与ＨＰＶ感染有密切关系。

Objective: To study the association of cervical cancer with human papillomaviral (HPV) infection in Uygur women at high risk for development of cervical cancer in Xinjiang. Method: Cervical samples from 65 Uygur women with cervical cancer were collected. HPV DNA types 6/11,16/18,31/33/35 were studied by in situ hybridization, and both consensus and type specific primers for HPV types 6,16 and 18 were used in 58 cases by polymerase chain reaction (PCR). Results: HPV DNA was detected in 43.1%, 22.4% and 77.6% of specimens by the in situ hybridization, PCR using L1 consensus primers and E6 type specific primers, respectively Conclusions: E6 type specific PCR was more sensitive than L1 consesus PCR and in situ hybridization for detection of HPV DNA. These data supported the role of HPV DNA (particularly HPV 16) in the pathogenesis of cervical carcinoma in high risk women living in Xinjiang.