nm23-H1提高舌癌细胞Tca8113对顺铂化疗敏感性可能机制的研究

Study of increased sensitivity on Tca8113 cell line to cisplatin by nm23-H1 in vitro

目的研究nm23-H1提高顺铂化疗敏感性的可能机制。方法实验分为nm23-H1转染组和未转染组,用MTT法检测顺铂对舌癌细胞的杀伤率;流式细胞仪检测细胞凋亡变化;用流式细胞仪检测线粒体结合罗丹明荧光强度,检测线粒体膜电位的变化;等离子质谱仪检测细胞内铂离子浓度变化。结果nm23-H1过表达可以明显提高顺铂对舌癌细胞的杀伤率,使细胞凋亡增强,线粒体膜电位降低,提高细胞内铂离子浓度。这种作用可以被哇巴因(Na+/K+-ATP酶的抑制剂)抑制。结论nm23-H1可提高顺铂对舌癌细胞化疗的敏感性,其可能机制是nm23-H1降低线粒体膜电位,铂离子进入细胞内增加,导致细胞凋亡或坏死。

Objective To study the mechanism of sensitivity variation to cisplatin caused by nm23-H1. Methods The samles was divided into two groups： TcaSll3 group and TcaSl13/nm23-H1 group. Using MTT and flow cytometer, the changes of cell mortality rate, apoptosis and mitochondrial membrane potential were detected. By VG PQ Excell, the changes of the intracellular platinum were detected. Results In vitro the cell mortality rate and apoptosis were increased in TcaSl13/nm23-H1 group, comparing with Tca8113 group. Mitochondrial membrane potential was decreased in TcaSl13/nm23-H1 group. The intracellular platinum was increased significantly in Tca8113/nm23-H1 group. This effect could be inhibited by oubain which was an inhibitor of Na^＋/K^＋-ATP. Conclusion nm23-H1 can increase the sensitivity of cisplatin on Tca8113 cell line. The mitochondrial membrane potential was decreased by nm23-H1 so that intracellular platinum was increased and finally increased the apoptosis or necrosis.