雌激素对大鼠咬肌热休克蛋白70表达的影响

Effect of estrogen on heat shock protein 70 expression in rat masseter muscle

目的探讨雌激素对大鼠咬肌热休克蛋白(HSP)70表达的影响。方法将60只雌性未孕12周龄Sprague-Dawley大鼠随机分成3组,每组20只。假手术组大鼠在轻微骚动卵巢后缝合,去卵巢组大鼠去除卵巢后缝合,雌激素替代组大鼠去卵巢后接受雌二醇替代治疗。在实验的第4周和8周,每组各处死10只大鼠,采用免疫组织化学方法研究雌激素对大鼠咬肌HSP70表达的影响。结果4周时3组大鼠咬肌HSP70的表达无明显差异(P>0.05);8周时去卵巢组大鼠HSP70的表达下降,与假手术组、雌激素替代组之间的差异有统计学意义(P<0.05),而假手术组与雌激素替代组之间的差异无统计学意义(P>0.05)。结论雌激素水平降低会导致大鼠咬肌HSP70的表达下降,雌激素替代疗法可以预防HSP70表达降低。

Objective To investigate the effect of estrogen on heat shock protein （HSP） 70 expression in rat masseter muscle. Methods Sixty twelve-week-old female Sprague-Dawley rats were randomly divided into three groups： Sham surgery group （control group）, ovariectomy group （OVX group）, ovariectomy with estradiol valerate replacement treatment group （OVX/EV group）. Half of the animals were sacrificed at 4 and 8 weeks respectively, then the masseter muscle was removed. Immunohistochemistry （IHC） method was employed to study the HSP70 expression in masseter muscle. Results Compared to control group and OVX/EV group, the expression of HSP70 was significantly lower at 8 weeks in OVX group （P〈0.05）. There were no significantly difference between the HSP70 expression of control group and that of OVX/EV group. Conclusion Estrogen may affect HSPT0 expression in rat masseter muscle, and estrogen replacement therapy may prevent HSP70 reduction.