人眼小梁细胞体外表达表皮生长因子mRNA和表皮生长因子受体

The expression of EGF mRNA and EGF receptors in human trabecular meshwork cells in vitro

目的了解培养的小梁细胞分泌表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）及细胞膜上表皮生长因子受体（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒｒｅｃｅｐｔｏｒ，ＥＧＦＲ）的情况。方法进行人眼小梁细胞体外培养。对传３～５代的小梁细胞，用鼠抗人ＥＧＦ单克隆抗体及免疫组化染色，检查小梁细胞ＥＧＦＲ情况。用ＥＧＦｃＤＮＡ探针，α－３２Ｐ同位素标记及斑点杂交放射自显影法，检测小梁细胞分泌ＥＧＦｍＲＮＡ的情况。结果人眼小梁细胞体外培养成功。免疫组化染色显示小梁细胞ＥＧＦＲ染色阳性，棕色反应颗粒位于细胞膜表面。斑点杂交结果阳性。结论小梁细胞能自身分泌ＥＧＦ。小梁细胞膜上同时存在ＥＧＦ受体。如果能选择性地上调小梁细胞膜受体或者促进小梁细胞分泌ＥＧＦ，可能对原发性开角型青光眼恢复小梁细胞功能，促进小梁细胞再生有一定意义。

Objective To demonstrate that cultured trabecular meshwork cells can secrete epidermal growth factor (EGF) and there are EGF receptors (EGFRs) on the cells. Methods Human trabecular meshwork cells were cultured in vitro and passaged 3 5 times. Immunohistochemical stain was used to detect EGFRs on the trabecular meshwork cell membrane. EGF cDNA probe, α 32 P isotope labeling and dot blot hybridization autoradiographic method were used to detect EGF mRNA in the cells. Results Cultured human trabecular meshwork cells were obtained. EGFR immunostain was positive, and the reaction of brown color was at the surface of the cells. Dot blot hybridization autoradiography showed that trabecular cells can express EGF mRNA. Conclusions Trabecular meshwork cells can secrete EGF. There are EGFRs on the membrane of trabecular meshwork cells. It is suggested that probably up regulating the receptors or promoting the cells to secrete growth factors have potential significance in the regeneration of trabecular cells and recovery of the cell functions in open angle glaucoma.