增殖细胞核抗原在人眼小梁细胞增殖研究中的应用

Application of proliferating cell nuclear antigen in the study of human trabecular cell proliferation

目的探讨增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）在细胞增殖研究中的应用价值。方法利用免疫组化方法对不同生长期（１２小时至２０天）的人眼第四代小梁细胞ＰＣＮＡ表达状况进行研究，并观察在不同浓度肾上腺素、地塞米松及表皮生长因子作用下ＰＣＮＡ的表达水平，并与正常细胞ＰＣＮＡ的表达水平进行对比研究，借助计算机图像处理系统对结果进行分析。结果根据正常细胞ＰＣＮＡ表达水平得到一稳定的细胞增殖曲线，该曲线可以明显表现出正常细胞生长的旺盛期及衰退期，可据此选择良好的药物作用时机以观察药物的敏感性。肾上腺素及地塞米松均明显抑制小梁细胞增殖，表皮生长因子则有明显的促增殖作用。结论ＰＣＮＡ可作为一种良好的观察细胞增殖状态的手段，有利于研究小梁细胞的其他生化特性及深入探讨原发性开角型青光眼的发病机制。

Objective To evaluate the application of proliferating cell nuclear antigen (PCNA) in the study of human trabecular cell proliferation. Methods Immunohistochemical technique was used to observe the PCNA expressive level in the fourth passage of human trabecular meshwork, the effects of different concentrations of epinephrine, dexamethasone and epidermal growth factor on the level were also investigated, the results were compared with that of the normal cells cultured at the same time, and analyzed by a graphic device operation system in a computer. Results A stable proliferating curve was obtained according to the normal cell PCNA level, by which we could choose the best opportunity of drug application. Epinephrine and dexamethasone were found to significantly inhibit cell proliferation, while epidermal growth factor (EGF) could promote the proliferation. Conclusion PCNA is considered to be a useful agent to observe the process of cell proliferation. The above mentioned methods are beneficial to the investigations of biochemical characteristics of trabecular meshwork cells and the pathogenic mechanisms of openangle glaucoma.