摘要
目的:探讨组织蛋白酶D(Cath-D)、肿瘤转移抑制基因表达蛋白(nm23-H1)的表达与食管癌临床病理特点及预后的关系。方法:应用免疫组织化学S-P法,以兔抗Cath-D、鼠抗nm23-H1抗体标记60例食管癌和5例正常的食管粘膜。观察不同分化程度和组织类型食管癌的表达情况,并比较其阳性率。结果:癌组织Cath-D阳性36例(60.0%),nm23-H1阳性35例(58.3%)。Cath-D的表达与癌组织分化程度、浸润深度、淋巴结转移和预后均无关(P>0.05);而nm23-H1蛋白的表达则与癌组织分化程度及淋巴结转移有关(P<0.05,P<0.01),与癌组织浸润深度和预后无关(P>0.05)。结论:nm23-H1可作为一种食管癌淋巴结转移的重要生物学标记物,但Cath-D、nm23-H1与预后的关系仍需进一步研究。
Purpose To determine the expression of cathepsin D (CathD) and metastasis suppressor gene protein (nm23H1) in esophageal carcinoma and its relationship to the clinicopathology and prognosis of the tumor. Methodds Sixty cases of esophageal carcinoma and 5 cases normal esophageal mucosa were stained by immunohistochemical SP method for CathD and nm23H1 protein antibody. The positivity rates in different differentiation and histologic type were compared statistically. Results Thirtysix cases (600%) and 35 cases (583%) of esophageal carcinoma were CathD and nm23H1 protein positive, respectively. The expression of CathD was not related to differentiation, invasiveness, lymph nodes metastasis and progression of the tumors (P>005) and the expression of nm23H1 protein was related to differentiation and to lymph nodes matastasis of the tumors (P<005,P<001), but not related to invasiveness and tumor progression (P>005). Conclusions The results suggest that nm23 might be an important biological marker in lymph node metastasis of esophageal carcinoma, but the association between CathD, nm23H1 and tumor progression should be further studied.
出处
《临床与实验病理学杂志》
CAS
CSCD
1997年第4期320-322,I032,共3页
Chinese Journal of Clinical and Experimental Pathology