HPLC法测定寒湿痹颗粒中马兜铃酸A

Determination the content of aristolochic acid A in Hanshibi Granules by HPLC

目的:建立测定寒湿痹颗粒中马兜铃酸A的高效液相色谱的分析方法。方法:色谱柱为Agilent Zorbax SB-C18柱(250mm×4.6mm,5μm),以甲醇-1%冰醋酸水溶液(55∶45)为流动相,流速为1.0ml/min,检测波长为315nm,建立HPLC检测方法。结果:马兜铃酸A峰面积与进样量在0.0102～0.1025μg范围内具有良好的线性关系(r=1.000)。平均回收率为99.3%(n=6),RSD=0.41%。本方法按信噪比为2∶1计算最低检测限为2ng。结论:该方法简单,可靠,检测限低,重现性好,可用于寒湿痹胶囊中马兜铃酸A的限量检测。

Objective： To establish a method for the determination of aristolochic acid A in Hanshibi Granules by HPLC Methods： The column was Agilent Zorbax SB-C18, mobile phase was methanol - 1% glacial acetic acid （55：45）.The flow rate was set at 1 ml/min,detection wavelength was set at 315 nm. Resultes： The calibration curve was linear at the range of 0.010 2-0.102 5 ug （r=1.000）. The average recovery was 99.3% （RSD=0.41%）. The limit of detection of aristolochic acid A was 2 ng. Conclusion： The method is simple and accurate, it can be used for determination of aristolochic acid A in Hanshibi Granules.