畜禽微卫星多态性的银染法显色

SILVER STAINING METHOD FOR MICROSATELLITE POLYMORPHISM OF THE DOMESTIC ANIMALS

为了确定一种安全、有效而快速的显示畜禽微卫星多态性的染色方法，以广东地方鸡种及优质黄羽肉鸡品系种鸡基因组ＤＮＡ为材料，用５对微卫星引物进行ＰＣＲ扩增，并将扩增产物置于变性聚丙烯酰胺凝胶中电泳分离，通过对比溴化乙锭染色和银染法显色确定理想显色法，结果认为银染法是一种可行、安全的理想方法。

or the purpose of founding the staining methods for revealing microsatellite polymorphism of domestic animals,PCR amplification was carried out with 5 pairs of microsatellite primers using genomic DNA of native chicken breeds as templates.Amplified products were separated by denaturing polyacrylamide gel electrophoresis.Comparing EB staining with silver staining,it was thought that silver staining was an available and safety method.