碱性蛋白酶水解玉米蛋白工艺条件的研究

Study on Alkaline Protease Hydrolysis of Corn Gluten Meal

通过单因素分析,对影响碱性蛋白酶水解玉米蛋白的主要因素进行了研究,利用正交试验筛选出最佳工艺参数。结果表明,酶水解最佳条件为底物浓度3%、酶浓度(E/S)3.0%、水解时间1h、温度45℃,在pH值为9.0条件下,水解度可达38.12%。

The factors affecting hydrolysis of corn gluten meal by alkaline protease were studied by mono-factor analysis, and the optimum technique parameters were determined by orthogonal test. The results showed that under the optimal conditions of： concentration of substrate 3%, enzyme to substrate ratio 3.0%, temperature 45 ℃, hydrolysis time 1 h and pH 9.0, the hydrolysis of degree is 38.12%.