气流粉碎对黑木耳蛋白溶出率和富集规律的影响

Effects of Vortex Grinding on Dissolving Ratio and Enrichment of Auricularia auricular Protein

本实验研究了黑木耳及其气流微粉中各自蛋白的最佳提取条件(黑木耳:pH7.6、30℃、2.5h、料液比1:45,气流微粉:pH7.4、35℃、料液比1:50、3h),在各自最佳条件下比较了黑木耳及其气流微粉蛋白的溶出率;并研究了振动筛分后不同粒度黑木耳气流微粉中蛋白质的富集规律。实验结果:气流微粉蛋白的溶出率平均比原料高2～3倍;筛分后100、140、180、200、240、300目黑木耳微粉中蛋白的溶出率随粒度减小而明显增大,蛋白主要富集在240～300目黑木耳微粉之间。

The extraction conditions of protein from rawAuricularia auricular and its vortex grinding micropowder were optimized by single test and orthogonal test. The optinmal extraction conditions of protein from the raw Auricularia auricular are as follows： pH 7.6, extraction temperature 30℃, time 2.5 h and ratio of material to water 1：45, and for the Auricularia auricular micropowder, those are as follows： pH 7.4, extraction temperature 35℃, time 3 h and ratio of material and water 1：50, Under the above optimal conditions, the dissolving ratio of protein from the raw Auricularia auricular was commpared with that of protein from its micropowed, and the protein enrichment regular of micropowed with different particle sizes after vibrating screen was investigated. It was found that the dissolving ratio of protein from the Auricularia auricular micropowed is 2 to 3 times higher than that of protein from the raw Auricularia auricula in average. The dissolving ratio of protein rises with the partein size of the Auricularia auricular micropowder declining in the range of 300, 240, 200, 180, 140 and 100 mesh, and the protein is enriched mainly in the Auricularia auricular micropowder between 240 mesh and 300 mesh.