两步法生产栀子蓝色素工艺条件的研究

Study on Production Technology of Gardenia Blue with Two-stage Method

本实验以栀子黄提取后的废液为原料,通过将β-葡萄糖苷酶发酵和酶促反应分开的两步法制得栀子蓝色素。分别考察了温度、时间和氨基酸的添加比例对栀子蓝生成量的影响。最后确定的最佳工艺条件为:以麸皮培养基固态发酵生产β-葡萄糖苷酶,浸提的β-葡萄糖苷酶液在60℃时水解栀子苷,在获得的栀子苷元溶液中添加1%的组氨酸,混和液再在80℃温度下水浴5h可以获得最大量的栀子蓝色素溶液。

Gardenia blue was made from exhausted solution of gardenia yellow by the two-stage method, in which β-glueosidase fermentation and enzymatic reaction are separated. The effects of temperature, time and added ratio of amino acid on gardenia blue formation. The results induced that the best production process of gardenia blue is as follows： producing β-glueosidase from bran solid medium by Aspergillus niger fermentation, then hydrolyzing gardenoside by the β-glucosidase at 60 ℃ to produce gardenosin. By adding 1% histidine into the gardenosin solution and then bathing the mixture at 80 ℃ for 5 h, and the maximum formation amount of gardenia blue is achieved.