多频超声连续逆流浸取黄芩中的黄芩苷

Multi-frequeny Ultrasonic Continuous Countercurrent Extraction of Baicalin from Scutellaria Root

本实验研究了单频、双频交变和双频复合超声连续逆流浸取黄芩苷的效果,为实现工艺优化,采用均匀设计试验方案,以浸取能力值为指标,用偏最小二乘法处理数据。结果表明,超声频率、功率和温度等关键影响因子单独和组合对不同超声连续逆流浸取效果的影响明显不同。优化的工艺条件为固液比1:5、70%乙醇、50℃、800W25+50kHz双频复合连续逆流浸取27min,浸取能力为5.3243g/L·min,比单频、双频交变连续逆流浸取分别提高18.6%和17.4%,是回流提取的492倍、超声间歇浸取的131倍。有明显技术优势和工业推广价值。

In this study, the baicalin was extracted from scutellaria root by single-frequency ultrasonic continuous countercurrent extraction （SUCCE）, dual-frequency-complex ultrasonic continuous countercurrent extraction （DCUCCE） and dual-frequency-alternant ultrasonic continuous countercurrent extraction （DAUCCE）. The experimental plans were done according to uniform design for the maximum exlraction of baicalin. The data were processed with PLS procedure and the effects of the factors, such as ultrasonic frequency, power and temperature on factor of extraction ability （FEA） ofbaicalin were confirmed by the standard regression coefficient. The optimum extraction conditions are determined as follow： solid and liquid rate 1：5, ethanol concenlration 70%, ultrasonic power 800 W, extraction time 27 rnin, extraction temperature 50 ℃; and DCUCCE frequency 25＋50kHz. Under the above conditions, FEA of 5.3243g/L.min, was obtained, which is well close to the predicted result, 18.6% and 17.4% more than that of SUCCE and DAUCCE respectively, 492 times and 131 times of that of classic extraction and ultrasonic intermittent extraction, respectively. Therefore, DCUCCE is worthy of using in industry.