和田玉枣总皂甙的提取工艺研究

Study on Extraction Process of Total Saponins from Hetian-jade Jujube

本实验以和田玉枣总皂甙含量为考察指标,以酸枣仁皂甙A为标准品,香草醛-高氯酸为显色剂,冰醋酸为溶剂,用紫外可见分光光度计在波长531nm处对和田玉枣总皂甙含量进行测定。最佳提取工艺为:提取温度70℃,乙醇浓度80%,料液比1:6,提取时间3h。该条件下,测得和田玉枣总皂甙含量为6.267mg/g。

The orthogonal design was used to optimize the extraction process of total saponins from hetian-jade jujube. With spine date seed saponin A as standard compound, vanillin-perclaloric acid as chromogenic reagent and glacial acetic acid as solvent, the determination method of the content of total saponins with UV spectrophotometer at 53 lnm was established. The results showed that the optimal extraction conditions are determined as follows： temperature 70 ℃, ethanol concentratin 80%, ratio of raw material to liquid 1：6 and extraction time 3 h. The content of total saponins in the crude extract from hetian-jade jujube is 6.267 mg/g.