姜黄素抗As_2O_3诱变作用的实验研究

Experimental Study of Inhibitive Effect of Curcumine Against Mutagenicity of As_2O_3

目的:研究姜黄素抗三氧化二砷(As2O3)的诱变作用。方法:分别采用小鼠骨髓细胞和人外周血淋巴细胞微核(MN)实验,计数含有MN的嗜多染红细胞和淋巴细胞数,计算MN率。观察As2O3细胞诱变性及不同浓度姜黄素对As2O3诱变性作用的影响。结果:①小鼠骨髓MN试验:与As2O3组比较,As2O3+1.2mg/mL,2.4mg/mL,4.8mg/mL姜黄素组小鼠骨髓细胞MN率显著降低(P<0.05,P<0.01)。②人外周血淋巴细胞MN试验:与空白对照组比较,1μg/mL、2μg/mL、4mg/mLAs2O3组人外周血淋巴细胞MN率升高(P<0.01);与1μg/mL(2μg/mL、4μg/mL)As2O3组比较,5μg/mL,10μg/mL,20μg/mL姜黄素+1μg/mL(2μg/mL,4μg/mL)As2O3组MN率降低(P<0.05,P<0.01)。结论:As2O3具有剂量相关的致突变作用,姜黄素具有明确的抗As2O3诱变性作用。

Objective ：To observe the inhibitive effect of curcumine against mutagenicity of As2O3. Methods： （1）Micronucleus （MN） frequency test in bone marrow of mice： 60 mice were randomly divided into blank control group, As2O3 group and 1.2 μg/mL,2.4 μ g/mL,4.8 μ g/mL curcumine＋As2O3 group （n=12）.Blank control group and As2O3 group were administered salad oil through intragastrical perfusion for 7 days.Then blank control group was injected intraperitoneally with 0.5 mL saline,other three groups were injected with 0.5 mL As2O3 solution （20 μ g/mL）.24 h later,mice were sacrificed and MN frequency test in bone marrow of mice was made. （2）MN frequency test in peripheral blood lymphocyte of human ： human blood samples were divided into 13 groups：blank control group,1 μg/mL,2 μg/mL,4 μg/mL As2O3 group,5 μg/mL,10 μg/mL,20 μg/mL cureumine＋1 μg/mL（2 μg/mL,4 μg/mL）As203 group.Then MN frequency test in peripheral blood lymphocyte of human was made to study mutagenieity of As2O3.Results： （1）Compared with As2O3 group, MN frequency in 1.2 mg/mL,2.4 mg/mL,4.8 mg/mL curcumine＋As2O3 group were significantly decreased （P〈0.05 ,P〈0.01 ）.（2） Compared with 1 μg/mL （2 μg/mL,4 μg/mL） As2O3 group,MN frequency in 5 μg/mL,10 μg/mL,20 μg/mL eurcum- ine＋1 μ g/mL （2 μ g/mL,4 μ g/mL） As2O3 group were significantly decreased （P〈0.05,P〈0.01）.Conclusion ：As2O3 has dosage-correlative mutagenic effect,and curcumine has a remarkable antimutagenic effect against As2O3.