柳杉试管快繁技术的研究

Studies on Mass Propagation in Vitro of Cryptomeria fortunei Hooibrenk ex Otto et Dietr by Tissue Culture

柳杉不仅是重要的高山速生树种,而且是一种优良的绿化和环保树种,是我国珍贵用材树种之一。以柳杉为材料,研究其组织培养快繁技术。结果表明:以柳杉种胚为外植体,在MS培养基中就可以得到很好的生长。侧芽的增殖能力要高于顶芽。柳杉侧芽茎尖培养在MS+ZT 0.3 mg/L培养基中,腋芽增殖系数最高达5.3,在MS+0.3 mg/L BA进行增殖培养,侧芽增殖系数达4.3。生根培养采用两阶段发根(先用IBA处理10 d,再转入1/3MS大量元素),发根率达80%,平均根数为5.25,平均根长为5.16 cm。试管苗经过驯化移栽成活率达85%以上。

Cryptomeriafortunei Hooibrenkex Otto et Dietr is not only an important fast-growing and precious species in forestry but also an evergreen and ecological conservative species in China. The techniques for micropropagation of Cryptomeria fortunei Hooibrenk were studied by tissue culture in this paper. The results indicated that the lateral shoot proliferation rate was up to 5.3 in MS ＋ 0.3 mg,/L ZT medium for mass propagation and obviously higher than that in MS ＋ 0.3 mg/L BA medium （4. 3）. The rooting rate of shoots was reached to 80% by two-step rooting method （first treated with 100 mg/ L IBA for 10 days, and then transferred into the 1/3 MS medium）. The average number of roots was 5.25 and the average length of roots was 5.16 cm. After acclimatization the survival rate of transplanted potted plants could be more than 85% with vigorous growing in green house.