中药方剂五苓散对SLT-2e影响大鼠肠黏膜微血管内皮细胞分泌NO的观察

Influence of Wulingsan and SLT-2e of Bacillus coli in the level of NO secreted by rat intestinal mucosal microvascular endothelial cells

将中药方剂五苓散和志贺样毒素Ⅱ型变异体(SLT-2e)分别加入大鼠肠黏膜微血管内皮细胞(RIM-VEC)培养板中培养1、3、6和9h,观察五苓散及其抗SLT-2e对RIMVEC分泌NO的影响。结果:普通中药组中10μL/mL五苓散浓度在1-9h内使NO浓度升高;治疗组五苓散浓度0.1μL/mL、1μL/mL、10μL/mL在9h时内皮细胞分泌NO差异不显著;预防组0.1μL/mL在1-6h时变化不明显,在9h时NO升高;1μL/mL、10μL/mL在6h时NO量分泌最高,9h时下降。提示一定量的五苓散可使RIMVEC的NO分泌量升高(P<0.05);治疗组五苓散使NO分泌量不升高,预防组五苓散可使内皮细胞分泌NO量升高(P<0.05)。

Traditional Chinese medicine Wulingsan and SLT-2e of Bacillus coli were added into medium to culture rat intestinal mucosal microvascular cells（RIMVECs） in vitro within 1,3,6 and 9 h.The results showed that 10uL/mL Wulingsan could inerease the level of NO secreted by RIMVEC within 1-9h in the group of common traditional Chinese medicine;While it was approximated on the level of NO secreted by RIMVEC among 0.1,1.0,10.0uL/mL wulingsan in treatment group;In the preventive group ,the level of NO was increased at 9 h with 0.1uL/mL Wulingsan,and NO showed peak value at 6h with 1.0 and 10.0uL/ML Wulingsan,It is concluded that Wulingsan could increase the level of NO secreted by RIMVEC（P〈0.05）,but could not effect the level of NO when RIMVEC was pretreated by SLT-2e of Bacillus coli.