湖南高致病性猪蓝耳病隐性感染情况调查

Epidemiological investigation on the latent infection with highly pathogenic porcine reproductive and respiratory syndrome virus

用ELISA和RT-PCR的方法对采集自湖南省内20个规模场1007份血清和3个市级定点屠宰场50份猪肺门淋巴结进行蓝耳病血清抗体检测和高致病性猪蓝耳病病毒的检测。结果1007份血清中,蓝耳病抗体阳性率为72.9%(734/1007),高致病性猪蓝耳病病毒携毒率为3.2%(32/1007),50份肺门淋巴结病毒阳性率为16%(8/50),其中,蓝耳病免疫与非免疫猪血清其抗体阳性率相差不显著,种猪的抗体阳性率明显高于商品猪,而其病毒携毒率为0%。部分规模猪场和眼观健康的育肥猪存在高致病性猪蓝耳病病毒的隐性感染。

1007 serum samples from 20 different pig farms and 50 lymphnode pulmonales from 3 slaughterhouses in Hunan province were detected for highly pathogenic porcine reproductive and respiratory ryndorme virus （HPPRRSV） by RT-PCR, and the serum antibody against PRRS were detected by ELISA. 72.9% serums were positive for PRRSV antibody （734/1007）, 3.2% serums（32/1007） and 16.0% lymphnode pulmonales （9/50） sample were positive for HP-PRRSV, the antibody positive percentage of the breeding stock was obviously higher than commercial swine, but no distinct difference between PRRS immune pig and not, there was＇t positive for HPPRRSV in the the serum samples of breeding stock.The results shows the antibody positive percentage of PRRS was high in different pig farms but the HP-PRRSV＇s latent infection existed in partial farms and healthy-in-appearance growing pigs.