3株肾综合征出血热病毒的分离和鉴定

Isolation and identification of 3 strains of hemorrhagic fever with renal syndrome virus

目的了解浙江省肾综合征出血热(HFRS)流行特点及汉坦病毒(HV)型别,为防治提供科学依据。方法鼠肺HV抗原检测采用直接免疫荧光法(DFA),病毒分离采用阳性鼠肺研磨液接种幼龄长爪沙鼠,再经Vero-E6细胞传代,用单克隆抗体DFA鉴定毒株及分型。结果从捕自浙江省龙泉地区的8份阳性鼠肺中分离到3株HV,经DFA鉴定均为汉滩型(Ⅰ型)病毒,3株病毒在Vero-E6细胞中的感染滴度为104.75～105.25CCID50/ml。结论从鼠类肺组织中分离到3株汉滩型HV,毒株均能在Vero-E6细胞中较好增殖,有可能成为HFRS疫苗后备毒株。

Objective To investigate the epidemiology of the hemorrhagic fever with renal syndrome（HFRS） and the serotype of hantavims（HV）. Methods Direct immunoflorscenee assay（DFA） was adopted to detect HFRS antigen and the vires was isolated from the young Meriones unguiculata inoculated with the rat lung tissues infected by HV. The isolated virus were continuously propagated in the Vero-E6 cell and identified by DFA using Anti-HV McAb. Results Three strains of HV were isolated from the eight lung tissues infected by HV which belonged to the hantaan type. The virus titer of three strains could reach 10475-105.25 CCID50/ml in the Veto-E6 cell. Conclusion Three strains of hantaan virus were successfully isolated from the lung tissue of rats, which could be the candidate strain for HFRS vaccine.