COPD患者支气管黏膜唾液酸转移酶mRNA表达水平的差异性

Variability of bronchial mucus membranes sialytransferases mRNA levels in patients with COPD

目的比较COPD和肺功能正常的肺癌手术患者相对正常的亚段以上支气管黏膜常见唾液酸转移酶mRNA表达水平的差异性。方法肺癌患者新鲜手术切除标本取远离肿瘤部位的亚段以上支气管黏膜,用RT-PCR方法检测4种黏蛋白常见唾液酸转移酶mRNA表达水平的高低。结果COPD和肺功能正常患者支气管黏膜的唾液酸转移酶3GalⅠ、Ⅲ、Ⅳ和唾液酸转移酶6GalⅠ的mRNA表达水平均无明显差异。结论COPD患者对细菌的易感染性及在急性加重期气道黏蛋白末端唾液酸结构的增多不是疾病特异性的,而更多是与感染直接相关。

Objective To investigate the variability of bronchial mucus membranes sialytransferases mRNA levels in the patients with lung cancer between complicated with COPD and with normal lung function and without chronic diseaseas of respiratory system. Methods After lung cancer exairesis,mucus membranes of bronchi far away from tumor were disconnected. The mRNA levels of four common mucins sialytransferase were analyzed with reverse transcription-polymerase chain raction. Results There were not different mRNA levels of bronchial mucus membranes sialytransferases 3 Gal Ⅰ , Ⅲ , Ⅳ and sialytransferases 6 Gal Ⅰ in the patients with COPD and with normal lung function. Conclusion It is much related to infection that infectibility to bacteria and increased terminal sialic acids of airway mucins during acute exacerbation of COPD,not COPD specificity.