线粒体膜电位在PUVA诱导HL-60、K562细胞凋亡时的变化

Effects of PUVA on Mitochondrial Membrane Potential in HL-60 and K562 Cells

目的:研究补骨脂素(PSO)加长波紫外线(UVA)光化学疗法(PUVA)诱导人白血病细胞HL-60、K562凋亡时对线粒体膜电位(ΔΨm)的影响。方法:细胞以不同浓度PSO,接受或不接受UVA照射后共同培养,电镜下观察细胞超微结构改变,流式细胞仪(FCM)检测细胞凋亡率和线粒体膜电位变化,采用多因素方差分析法进行统计学处理。结果:PUVA处理后的HL-60、K562细胞超微结构出现明显的凋亡形态学改变;PSO、UVA照射及PUVA均可使细胞凋亡率增加,ΔΨm下降,PUVA的作用显著强于前两者(P<0.01)。结论:PUVA可诱导人白血病细胞发生凋亡,诱导凋亡的途径之一为下调线粒体膜电位水平。

Objective ：To study the effects of psoralen（PSO） and long wave ultraviolet light （PUVA） on mitochondrial membrane potential in HL-60 and K562 cells. Methods： Cells were incubated with PSO in different concentrations irradiated with or without UVA. The changes of ultrastructure of cells were observed under the electron microscope. The apoptosis ratios and the changes of mito- chondrial membrane potential were detected through the flow cytometry. The factorial design and analysis of variance were used to analyze the interaction among the factors. Results：There were obvious ultrastructure changes about apoptosis in HL-60 and K562 cells after treated with PUVA. PSO, UVA and PUVA all increased the apoptosis ratios and decreased the mitochondrial membrane potential, and the effects of PUVA were stronger than the other two（ p 〈0. 01 ）. Conclusion：PUVA can induce the apoptosis of HL-60 and K562 cells and one of the pathway to induce apoptosis is to downregulate the mitochondrial membrane potential.