糙叶败酱提取物对荷瘤小鼠血清细胞因子水平的影响

Effect of Patrinia scabra Extracts on the Level of Serum Cytokine in Sarcoma 180 Ascitic Tumor Burdened Mice

目的:观察糙叶败酱提取物对Sarcom a 180腹水瘤荷瘤小鼠血清细胞因子水平的影响,初步探讨其抗肿瘤作用机制。方法:建立小鼠腹水瘤模型,采用糙叶败酱提取物高(2.0 g/kg)、中(1.0 g/kg)、低(0.5 g/kg)剂量腹腔注射治疗,1次/d,连续10 d。治疗结束后,采集小鼠血清,用双抗体夹心ELISA法检测血清Th1型细胞因子IL-2、IL-12、IFN-γ、TNF-α和Th2型细胞因子IL-6、IL-10的水平。结果:高剂量组血清IL-2、IFN-γ含量升高,与模型组比较,差异显著(P<0.05)。高、中剂量组血清IL-6和IL-10含量降低,与模型组比较差异显著(P<0.01)。结论:糙叶败酱提取物能促进Th1型细胞因子IL-2、IFN-γ的产生,同时抑制Th2细胞因子IL-6和IL-10分泌,通过增强抗肿瘤免疫反应而发挥抗肿瘤作用。

Objective ：To explore the effect of Patrinia scabra extracts （PSE） on the level of serum cytokine in Sarcoma 180 ascitic tumor burdened mice and its mechanism of anti-tumor. Methods ：The mice model of Sareoma 180 ascitic tumor were established and divided into five groups randomly, including the model group with normal saline solution, the positive group with 10mg/kg cytoxan and PSE treated groups at doses of 2. 0 g/kg, 1.0 g/kg,0. 5 g/kg intraperitoneally for 10 days. The level of serum cytokine Thl （ IL-2, IL- 12,IFN-γ,IFN-α and Th2 （IL-6, IL-10） were measured by double antibody sandwich ELISA assay. Results：Compared with model group of Sarcoma 180 ascitic tumor burdened mice,the level of IL-2 and IFN-γ, increased in PSE 2.0 g/kg group,but the IL-6 and IL- 10 decreased in PSE 2. 0 g/kg and 1.0mg/kg groups. Conclusion ： PSE has anti-tumor effect in vivo that could be related to the level variation of IL-2,IFN-γ,IL-6 and IL-10 in tumor burdened mice.