酸枣仁对阴虚小鼠焦虑行为的影响

Effects of Semen Ziziphi Spinosae on the Anxiety Behavior of the Yin Deficiency Mice

目的:观察酸枣仁对阴虚小鼠焦虑行为的影响,筛选活性物质并探讨其抗焦虑机制。方法:采用320mg/kg甲状腺片混悬液给小鼠连续灌胃9 d造阴虚模型,以高架十字迷宫实验和明暗箱实验观察阴虚模型小鼠焦虑行为状态以及酸枣仁的干预作用。实验后立即取脑,薄层扫描法测定脑GABA和Glu的含量;免疫组化法观察GABAAR1和NMDAR1的表达。结果:①与阴虚模型组比较,地西泮组和醇提物中剂量组小鼠在高架十字迷宫实验中进入开臂的时间比和次数比显著增加(P<0.05),在明暗箱实验中穿箱次数显著增加(P<0.05)。②与阴虚模型组比较,地西泮组和醇提物中剂量组小鼠脑内GABA含量显著升高(P<0.05)、Glu含量显著降低(P<0.05)。③与阴虚模型组比较,地西泮组和醇提物中剂量组小鼠脑内GABAAR1平均积分光密度值显著升高(P<0.05)、NMDAR1平均积分光密度值显著降低(P<0.05)。结论:酸枣仁醇提物具有明显的抗焦虑作用。其抗焦虑机制可能与提高小鼠脑内GABA含量,增强GABAAR1表达,降低Glu含量和NMDAR1表达有关。

Objective ：To observe the effects of Semen Ziziphi Spinosae on the anxiety behavior of the yin deficiency mice, to filter the active material of Semen Ziziphi Spinosae and explore its anti-anxiety mechanism. Methods：The yin deficiency mice model with intragastrieal 320mg/kg thyroid tablet for nine days exception normal control group. To observe yin deficiency model mice anxiety action by means of the elevated plus-maze and light-dark transitions of mice. After the experiment, peeled off cerebra at once. The left saved in liquid nitrogen for assaying GABA and Glu with TLCS,and right saved in formalin for the protein expression of GABAAR1 and NMDARl with immunohistochemical method. Resuhs：①Compared with yin deficiency model group, Tabellae Diazepami group and the middle alcohol extracts group increased instinctively in the percentage of time and degree entering open-arm in eleveated plus maze, and the passing times in light-dark transitions increased instinctively （ P 〈 0.05 ）. ②Compared with yin deficiency model group, the contents of GABA increased evidently（ P 〈 0. 05 ） and Glu reduced evidently （ P 〈 0. 05 ） in Tabellae Diazepami group and the middle alcohol extracts group. ③Compared with yin deficiency model group,the expression of GABAAR1 increased evidently（ P 〈0. 05） and NMDAR1 reduced evidently（ P 〈0.05） in the diszepam group and the middle alcohol extracts group. Conclutions：The alcohol extracts of Semen Ziziphi Spinosae have the anti-anxiety effects instinctively. Its mechanism may be related to increasing the GABA and expression of GABAA R1 and reducing the Glu and expression of NMDAR1.