高侵袭和EGFR过表达的人脑胶质瘤组织异种原位移植模型

Establishment of orthotopic xenograft model of human brain glioma with high invasiveness and overexpression of EGFR

目的建立能向脑组织侵袭和过表达表皮生长因子受体（EGFR）的人脑胶质瘤裸小鼠原位移植模型。方法首先将体外培养高表达EGFR的人脑多形性胶质母细胞瘤细胞行裸小鼠右尾状核接种，继而将致瘤的组织块行鼠到鼠的原位传代接种。结果鼠－鼠原位传代接种至13代，生存期为（19±1．33）d。移植瘤病理符合高侵袭、高表达EGFR的多形性胶质母细胞瘤。肿瘤增殖潜伏期短于3d，快速增殖期长于15d，晚期短于3d。结论肿瘤组织块接种比单细胞悬液接种、非但接种的细胞量大，还能将支持肿瘤细胞增殖的间质成分一并植入，有利于移植瘤保持亲本肿瘤的高侵袭、高表达EGFR的特征。

Objective To establish orthotopic xenograft model of human brain glioma in nude mice with characteristics of high invasiveness and overexpression of EGFR. Method Human glioblastoma muhiforme cell line with overexpression of EGFR was cultured in vitro, then tumor cells were injected stereotaxically into the right caudate nucleus of nude mice. The newly formed solid tumor were sharply dissected and 2.0mm^3 tumor tissue were implanted in the fight caudate nucleus of nude mice. Results The xenografts have been passed continuously in 130 mice for 13 generations in caudate nucleus of nude mice, and the survival time were （ 19 ± 1.33 ） days. Histological assay suggests the grafts were high invasive with overexpression of EGFR ,which were in accordance with the characteristics of the original human glioblastoma muhiforme. The tumor proliferation curve suggests that tumor latent period was less than 3 days, rapid proliferation phase were longer than 15 days, and the advanced stage to dead point was less than 3 days. Conclusions Compared with tumor cells suspension directly innoeulated into the caudate nucleus of nude mice, trausplantion of solid tumor tissue took on the advantages of not only injection of plenty of tumor cells, but also tumor matrix that were necessary for the rapid tumor growth, which was helpful for maintaining the parent tumor with high invasiveness and overexpression of EGFR.