摘要
[目的]探讨大豆皂甙(Soyasaponin,SS)对实验性大鼠矽肺纤维化模型中肺组织细胞凋亡的影响。[方法]通过复制大鼠矽肺纤维化模型,观察各组大鼠血清中一氧化氮(NO)、活性氧族(ROS)在染尘及SS干预后的变化情况。并采用TUNEL法观察各组大鼠肺组织细胞凋亡的形态学变化及凋亡指数的改变。[结果]SS干预后,血清NO、ROS与对模型组相比均显著降低(P﹤0.05)。TUNEL法显示大鼠肺组织细胞凋亡指数有所下降(P﹤0.05),但镜下仍可见肺组织内肺泡上皮细胞、肺血管内皮细胞呈凋亡改变。[结论]一定剂量的大豆皂甙可以通过减少肺组织细胞的过度凋亡,从而缓解矽肺纤维化的进展。
[Objectives] To discuss effect of SS on pulmonary tissue apoptosis of silicotie fibrosis rats. [Methods] Observed nitrous oxide （NO） and reactive oxygen species （ROS） in serum and changes after intervention of SS. Analysis of apoptosis of pulmonary tissue cells： apoptosis was detected by TdT-mediated dUTP nick end labeling （TUNEL） to determine the changes of morphology and apoptotic index. [ Results] After intervention of SS, the level of ROS, NO were all decreased significandy （P 〈 0.05）. TUNEL showed that apoptotic index descented remarkably. But we could also observe the appearance of apoptosis in alveolar epithelial ceils and pulmonary vascular endothehal cells. [Conclusion] A certain dose of SS might reheve the progress of silicotic pulmonary. fibrosis through reducing excessive apoptosis of puhnonary tissue.
出处
《现代预防医学》
CAS
北大核心
2008年第23期4681-4683,共3页
Modern Preventive Medicine
关键词
矽肺
肺纤维化
大豆皂甙
细胞凋亡
Silicosis
Pulmonary fibrosis
Soyasaponin
Apoptosis
