雷公藤制剂中雷公藤红素的高效液相色谱法测定

Determination of Tripterine in Tripterygium Wilfordii Hook f. by HPLC

文章采用外标法建立雷公藤制剂中雷公藤红素的HPLC测定方法．色谱柱：Agilent ZORBAX SC—C18（4．6mm×25cm，5μm），流动相。甲醇-1％醋酸溶液（87：13）；检测波长；425nm，流速：1．0mL／min，进样量：20μL，柱温；25℃。雷公藤红素在40．96-204．8μg／mL之间呈良好的线性关系，r=0．9996，n=5，平均回收率为98．97％，RSD=1．33％（n=6）。实验方法具有操作简便、准确，回收率好，精密度、重现性高，分析快速的优点，可作为雷公藤饼剂中雷公藤红索的定量分析和质量控箭方法。

Determine the content of tripterine in Tripterygium wilfordii Hook f. by HPLC method. An external standard method was used with Angilent Zorbax SC--C18 and the mobile phase of Methanol：1% HAc（87：13）. The wavelength for the detection was 425 nm and the flow rate was 1.0mL/min. temperature： 25℃. The linear range for the determination of tripterine was 40.96-204.8/lg/mL （r =0. 9996, n=5）. The average recovery was 98.97% and RSD was 1.33%（n=6）. The method is simple and accurate, good separated degree, the results are sensitive and reproducible and may be used in the quality control of tripterine in Tripterygium wilfordii Hook f suppositories.