FABP2多态性与2型糖尿病胰岛素抵抗、糖、脂代谢的关系

Relationship between polymorphism of the human intestinal fatty acid binding protein gene and metabolic parameters of type 2 diabetic patients

目的:探讨小肠脂肪酸结合蛋白基因(FABP2)多态性与2型糖尿病(T2DM)胰岛素抵抗、糖、脂代谢的关系。方法:应用聚合酶链反应,对140例蚌埠地区汉族人80例2型糖尿病(T2DM)组及60例正常对照(NC)组的小肠脂肪酸结合蛋白基因54密码子的HhaⅠ酶切位点进行限性片段长度多态性分析。结果:蚌埠地区汉族人存在FABP2HhaⅠ多态性位点,有FABP2Ala54Ala、Ala54Thr和Thr54Thr多态片段(等位基因频率Ala为0.67,Thr为0.33)。与NC组比较,T2DM组的胰岛素抵抗指数(HOMA-IR指数)、胰岛素均明显增高(P<0.01),胰岛素敏感指数显著降低(P<0.01);甘油三酯、总胆固醇、低密度脂蛋白均明显增高(P<0.01),高密度脂蛋白明显降低(P<0.01)。T2DM组中,FABP2Ala54Ala与Ala54Thr及Thr54Thr基因型患者比较,后两者的空腹胰岛素、HOMA-IR指数、空腹血糖、甘油三酯水平、低密度脂蛋白明显增高(P<0.01),而胰岛素敏感指数显著降低(P<0.01)。结论:蚌埠地区汉族人存在FABP2多态性。2型糖尿病患者存在胰岛素抵抗及脂代谢异常,且FABP2Ala54Thr、Thr54Thr基因型与胰岛素抵抗相关,是脂代谢异常的影响因素之一。

Objective：To explore relationship between polymorphism of the human intestinal fatty acid binding protein gene and metabolic parameters of type 2 diabetic patients.Methods：One hundred and fourty Han nationality in Bengbu were screened for presence of codon 54 variation by PCR-RFLP assay,identified by the enzyme HhaⅠ[Eighty patients with type 2 diabetes mellitus（T2DM）and sixty normal controls（NC）].Results：The polymorphic restriction site was found in the FABP 2 identified by the enzyme HhaⅠin Han nationality of Bengbu（the freqencies of Ala54 and thr54 in Bengbu were 0.67and 0.33,respectively）.The type 2 diabetes-mellitus（T2DM）subjects had higher HOMR-IR,FINS,FPG,TC,TG,LDL and lower ISI than NC subjects.The 2DM subjects with genotype Thr54（＋）（Thr54 homozygotes and heterozygotes）had higher FPG、FINS、HOMR-IR、TG、LDL（P〈0.01）and lower ISI（P〈0.01）than those with genotype Thr（-）（Ala54 homozygotes）.Conclusions：The polymorphy of FABP2 has been identified in the Han nationality in Bengbu.The genotype Thr54 homozygotes and heterozygotes of FABP2 gene seems to be associated with the insulin resistance of the type 2 diabetic patients.They also may be involved in the determination of lipoprotein profiles in the type 2 diabetic patients.