酸浆的组织培养与植株再生

Tissue Culture and Plantlet Regeneration of Physalis alkekengi L.

以酸浆的叶片、叶柄、茎段、子叶、胚轴、胚根为外植体进行离体培养,结果表明:叶柄为酸浆不定芽分化的最佳外植体类型;IAA为诱导叶柄不定芽分化较适合的生长素类型;不定芽分化的最佳培养基为MS+2.0 mg/L6-BA+0.5 mg/L IAA;丛生芽增殖的最佳培养基为MS+2.0 mg/L 6-BA+0.05 mg/L IAA,增殖系数达5.0;最优的生根培养基为1/2 MS,生根率达91.7%.

The leaves, petiole, stems, cotyledon, embryonic axis and radicle of Physalis alkekengi as explants were cultured in vitro. The results were as follows. For inducing adventitious buds, petiole was the optimal explant; IAA was the best growth hormone; and MS＋2.0 mg/L 6-BA＋0.5 mg/L IAA was the most appropriate hormone mixture; MS ＋ 2. 0 mg/L 6-BA ＋ 0. 05 mg/L IAA was the most appropriate media for differentiation and multiplication of adventitious shoots; the optimum rooting medium was 1/2 MS, and its efficiency was 91.7%.