趋化因子9在口腔黏膜下纤维化和口腔扁平苔藓中的表达及意义

Expression of CXCL9 in oral submucous fibrosis and oral lichen planus

目的:探讨趋化因子9(CXCL9)在口腔黏膜下纤维化(OSF)和口腔扁平苔藓(OLP)中的表达以及在炎症和免疫反应中意义。方法:采用免疫组化技术检测66例OSF、17例OLP和10例正常颊黏膜中CXCL9蛋白的表达及定位情况,采用Western blotting定量检测OSF颊黏膜、OLP颊黏膜和正常颊黏膜中CXCL9蛋白表达。结果:43例(65.2%)OSF和16例(94.1%)OLP淋巴细胞和内皮细胞胞质呈CXCL9强阳性表达,而在正常组中CXCL9呈阴性表达。OSF和OLPCXCL9阳性表达率显著高于正常组,而OLPCXCL9阳性表达率明显高于OSF(χ2=4.20,P<0.05)。CXCL9蛋白在OSF中的阳性表达率与病理分期无关(χ2=3.84,P>0.05)。Western blotting结果显示CXCL9蛋白表达水平在正常颊黏膜、OSF和OLP中依次增高,差异有统计学意义(P<0.05)。结论:CXCL9在淋巴细胞和炎症细胞的趋化、聚集以及OLP和OSF发生发展中发挥了重要作用。

Objective：To investigate the expression of CXCL9 in oral submucous fibrosis （OSF） as well as oral lichen planus （OLP） and its role in the inflammatory and immunological reaction. Methods ：The expression of CXCL9 was examined in 66 OSF specimens, 17 OLP specimens and 10 normal buccal mucosa tissues by immunohistochemistry, and the protein level of CXCL9 were detected by Western blotting. Results： 43 （65.2%） cases of OSF and 16 （94.1%） cases of OLP showed positive immunoreactivity for CXCL9 in the cytoplasm of inflammatory cells and endothelial cells. All normal buccal mucosa tissues stained negative for CXCLg. The expression levels of CXCL9 in OLP and OSF were significantly higher than that of normal controls, and the expression level in OLP was significantly higher than in OSF（X^2 = 4.20 ,P 〈 0.05 ）. Western blotting results were fully consistent with the immunohistochemieal data. Conclusion：CXCL9 might play vital roles in the pathopoiesis of OSF and OLP.